Description
Description: This assay employs a two-site sandwich ELISA to quantitate FEM1C in samples. An antibody specific for FEM1C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FEM1C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FEM1C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FEM1C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: FEM1C contains 6 ankyrin repeats and shares 65% amino acid identity with mouse Fem1a and 34% identity with C. elegans Fem1, a protein involved in nematode sex determination. Northern blot analysis detected transcripts of about 4.5 and 7.5 kb in all tissues examined, with highest abundance in heart and kidney. Lower expression was detected in brain, skeletal muscle, lung, liver, placenta, and cartilage. RT-PCR detected FEM1C in all tissues examined.
The deduced 617-amino acid protein shares 41% identity with FEM1B, an ankyrin repeat protein. RT-PCR ELISA detected intermediate expression in all adult and fetal tissues and specific brain regions examined except lung, which showed little to no expression